Publications and Presentations from the NIST Forensics/Human Identity Project Team

Team Members: John M. Butler (Project Leader), Margaret C. Kline, Peter M. Vallone, Michael D. Coble, Janette W. Redman, Amy E. Decker,  Becky Hill, Chris DeAngelis, David L. Duewer (Analytical Chemistry Division)

$Funding from the National Institute of Justice through the NIST Office of Law Enforcement Standards

[Publications]    [Presentations]   [NEAFS workshop materials on CE and STRs]

Publications (click on the publication to download the pdf)

2005

Coble, M.D. and Butler, J.M. (2005) Characterization of new miniSTR loci to aid analysis of degraded DNA. J. Forensic Sci. 50: 43-53.

Butler, J.M. (2005) Constructing STR multiplex assays. Methods in Molecular Biology: Forensic DNA Typing Protocols (Carracedo, A., ed.), Humana Press: Totowa, New Jersey, 297: 53-66. [preprint]

 

Vallone, P.M., Fahr, K., Kostrzewa, M. (2005) Genotyping SNPs using a UV photocleavable oligonucleotide in MALDI-TOF MS. Methods in Molecular Biology: Forensic DNA Typing Protocols (Carracedo, A., ed.), Humana Press: Totowa, New Jersey, 297: 169-178.

Vallone, P.M., Decker, A.E., Butler, J.M. (2005) Allele frequencies for 70 autosomal SNP loci with U.S. Caucasian, African American, and Hispanic samples. Forensic Sci. Int. 149: 279-286.

Kline, M.C., Vallone, P.M., Redman, J.W., Duewer, D.L., Calloway, C.D., Butler, J.M. (2005) Mitochondrial DNA typing screens with control region and coding region SNPs. J. Forensic Sci. 50: 377-385.

Kline, M.C., Duewer, D.L., Redman, J.W., Butler, J.M. (2005) Results from the NIST 2004 DNA Quantitation Study. J. Forensic Sci., 50(3): 571-578.

Brettell, T.A., Butler, J.M., Saferstein, R. (2005) Forensic science. Anal. Chem. 77: 3839-3860.

Butler, J.M., Appleby, J.E., Duewer, D.L. (2005) Locus-specific brackets for reliable typing of Y-chromosome short tandem repeat markers. Electrophoresis, 26: 2583-2590.

Butler, J.M., Decker, A.E., Kline, M.C., Vallone, P.M. (2005) Chromosomal duplications along the Y-chromosome and their potential impact on Y-STR interpretation. J. Forensic Sci., 50(4): 853-859.

Butler, J.M. and Schoske, R. (2005) U.S. population data for the multi-copy Y-STR locus DYS464. J. Forensic Sci. 50(4): 975-977.

Butler, J.M., Decker, A.E., Vallone, P.M., Kline, M.C. (2005) Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samples. Forensic Sci. Int., in press. [preprint]

Menotti-Raymond, M.A., David, V.A., Wachter, L.A., Butler, J.M., O’Brien, S.J. (2005) An STR forensic typing system for genetic individualization of domestic cat (Felis catus) samples. J. Forensic Sci., in press. [preprint]

Gusmão, L., Butler, J.M., Carracedo, A., Gill, P., Kayser, M., Mayr, W.R., Morling, N., Prinz, M., Roewer, L., Tyler-Smith, C., Schneider, P.M. (2005) DNA Commission of the International Society of Forensic Genetics (ISFG): An update of the recommendations on the use of Y-STRs in forensic analysis. Forensic Sci. Int., in press. [preprint]

Butler, J.M. (2005) High-throughput genotyping of short tandem repeat DNA markers with time-of-flight mass spectrometry. Encyclopedia of Mass Spectrometry, Volume 3, in press.

Vallone, P.M. and Butler, J.M. (2005) Analysis of DNA single nucleotide polymorphisms by mass spectrometry. Encyclopedia of Mass Spectrometry, Volume 3, in press.

Niederstätter, H., Coble, M.D., Grubwieser, P., Parsons, T.J., Parson, W. (2005) Characterization of mtDNA SNP typing and mixture ratio assessment with simultaneous real-time PCR quantification of both allelic states. Int. J. Legal Med., in press [preprint].

Butler, J.M. (2005) Genetics and genomics of core STR loci used in human identity testing, submitted.

Dixon, L.A., Dobbins, A.E., Pulker, H., Butler, J.M., Vallone, P.M., Coble, M.D., Parson, W., Berger, B., Grubweiser, P., Mogensen, H.S., Morling, N., Nielsen, K., Sanchez, J.J., Petkovski, E., Carracedo, A., Sanchez-Diz, P., Brion, M., Irwin, J.A., Just, R.S., Loreille, O., Parsons, T.J., Syndercombe-Court, D., Schmitter, H., Gill, P. (2005) Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise, submitted.

Biesecker, L.G., Bailey-Wilson, J.E., Ballantyne, J., Baum, H., Bieber, F.R., Brenner, C., Budowle, B., Butler, J.M., Carmody, G., Conneally, P.M., Duceman, B., Eisenberg, A., Forman, L., Kidd, K.K., LeClair, B., Niezgoda, S., Parsons, T., Pugh, E., Shaler, R., Sherry, S.T., Sozer, A., Walsh, A. (2005) DNA identification of victims from the September 11, 2001 World Trade Center attack: scientific challenges and policy implications, submitted.

 

2004

Butler, J.M. and Vallone, P.M. (2004) High-throughput genetic analysis through multiplexed PCR and multicapillary electrophoresis. PCR Technologies: Current Innovations (2^nd edition), Weissensteiner, T., Griffin, H.G., Griffin, A. (Eds.), CRC Press: Boca Raton, Chapter 11, pp 111-120.

Schoske, R., Vallone, P.M., Kline, M.C., Redman, J.W., Butler, J.M. (2004) High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assays, Forensic Sci. Int. 139: 107-121.

Edson, S.M., Ross, J.R., Coble, M.D., Parsons, T.J., and Barritt, S.M. (2004) “Naming the dead — confronting the realities of rapid identification of degraded skeletal remains.” Forensic Science Reviews 16(1): 64-89.

 

Vallone, P.M. and Butler, J.M. (2004) Multiplexed assays for evaluation of Y-SNP markers in U.S. populations. Progress in Forensic Genetics 10, Elsevier Science: Amsterdam, The Netherlands, International Congress Series 1261, 85-87.

 

Butler, J.M. and Schoske, R. (2004) Forensic value of the multi-copy Y-STR marker DYS464. Progress in Forensic Genetics 10, Elsevier Science: Amsterdam, The Netherlands, International Congress Series 1261, 278-280.

Butler, J.M., Buel, E., Crivellente, F., McCord, B.R. (2004) Forensic DNA typing by capillary electrophoresis: using the ABI Prism 310 and 3100 Genetic Analyzers for STR analysis. Electrophoresis, 25: 1397-1412.

Vallone, P.M., Just, R.S., Coble, M.D., Butler, J.M., Parsons, T.J. (2004) A multiplex allele-specific primer extension assay for forensically informative SNPs distributed throughout the mitochondrial genome. Int. J. Legal Med., 118: 147-157.

Coble, M.D., Just, R.S., O'Callaghan, J.E., Letmanyi, I.H., Peterson, C.T., Irwin, J.A., Parsons, T.J. (2004) Single nucleotide polymorphisms over the entire mtDNA genome that increase the power of forensic testing in Caucasians. Int. J. Legal Med., 118: 137-146.

Coble, M.D. (2004) The identification of single nucleotide polymorphisms in the entire mitochondrial genome to increase the forensic discrimination of common HV1/HV2 types in the Caucasian population. PhD dissertation, George Washington University, 206 pp. 

Butler, J.M. and Schoske, R. (2004) Duplication of DYS19 flanking regions in other parts of the Y chromosome. Int. J. Legal Med., 118: 178-183.

Vallone, P.M. and Butler, J.M. (2004) Y-SNP typing of U.S. African American and Caucasian samples using allele-specific hybridization and primer extension. J. Forensic Sci. 49(4): 723-732.

Chung, D.T., Drabek, J., Opel, K.L., Butler, J.M., McCord, B.R. (2004) A study on the effects of degradation and template concentration on the efficiency of the STR miniplex primer sets. J. Forensic Sci. 49(4): 733-740.

Drabek, J., Chung, D.T., Butler, J.M., McCord, B.R. (2004) Concordance study between miniplex STR assays and a commercial STR typing kit, J. Forensic Sci. 49(4): 859-860.

Vallone, P.M. and Butler, J.M. (2004) AutoDimer: a screening tool for primer-dimer and hairpin structures. Biotechniques, 37(2): 226-231.

Butler, J.M. (2004) Short tandem repeat analysis for human identity testing. Current Protocols in Human Genetics, John Wiley & Sons, Hoboken, NJ, Unit 14.8, (Supplement 41), pp. 14.8.1-14.8.22 [preprint]

 

Butler, J.M., Tomsey, C.S., Kline, M.C. (2004) Can the validation process in forensic DNA typing be standardized? Proceedings of the 15^th International Symposium on Human Identification. Available at  http://www.promega.com/geneticidproc/ussymp15proc/oralpresentations/butler.pdf  or http://www.cstl.nist.gov/biotech/strbase/pub_pres/PromegaPaperOct2004.pdf.

Duewer, D.L., Kline, M.C., Redman, J.W., Butler, J.M. (2004) NIST Mixed Stain Study #3: signal intensity balance in commercial short tandem repeat multiplexes, Anal. Chem. 76: 6928-6934.

Just, R.S., Irwin, J.A., O'Callaghan, J.E., Saunier, J.L., Coble, M.D., Vallone, P.M., Butler, J.M., Barritt, S.M., Parsons, T.J. (2004) Toward increased utility of mtDNA in forensic identifications. Forensic Sci. Int. 146S: S147-S149.

 

2003

Schoske, R., Vallone, P.M., Ruitberg, C.M., Butler, J.M. (2003) Multiplex PCR design strategy used for the simultaneous amplification of 10 Y chromosome short tandem repeat (STR) loci. Anal. Bioanal. Chem., 375: 333-343.

Butler, J.M., Schoske, R., Vallone, P.M. Highly multiplexed assays for measuring polymorphisms on the Y-chromosome. (2003) Progress in Forensic Genetics 9 (Brinkmann, B. and Carracedo, A., eds.), Elsevier Science: Amsterdam, The Netherlands, International Congress Series 1239, pp. 301-305.

Schoske, R. (2003) The design, optimization and testing of Y chromosome short tandem repeat megaplexes. PhD dissertation, American University, 270 pp.

Butler, J.M., Schoske, R., Vallone, P.M., Redman, J.W., Kline, M.C. (2003) Allele frequencies for 15 autosomal STR loci on U.S. Caucasian, African American, and Hispanic populations. J. Forensic Sci. 48(4):908-911.

Kline, M.C., Duewer, D.L., Redman, J.W., Butler, J.M. (2003) NIST mixed stain study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity. Anal. Chem. 75: 2463-2469.

 

Butler, J.M. (2003) Recent developments in Y-short tandem repeat and Y-single nucleotide polymorphism analysis. Forensic Sci. Rev. 15:91-111.

 

Kline, M.C., Duewer, D.L., Butler, J.M. (2003) Biotech SRMs designed for the rapidly evolving forensic DNA and human identity testing communities. NCSL International 2003 Workshop and Symposium Conference Proceeding: The Spectrum of Metrology: From the State-of-the-Art to the Everyday. See http://www.ncsli.org

 

Levin, B.C., Holland, K.A., Hancock, D.A., Coble, M., Parsons, T.J., Kienker, L.J., Williams, D.W., Jones, M.P., and Richie, K.L. (2003) “Comparison of the complete mtDNA genome sequences of human cell lines - HL-60 and GMIO742A - from individuals with pro-myelocytic leukemia and leber hereditary optic neuropathy, respectively, and the inclusion of HL-60 in the NIST human mitochondrial DNA standard reference material - SRM 2392-I.” Mitochondrion 2: 387-400.

 

Butler, J.M., Shen, Y., McCord, B.R. (2003) The development of reduced size STR amplicons as tools for analysis of degraded DNA. J. Forensic Sci 48(5) 1054-1064.

 

2002

Kline, M.C., Duewer, D.L., Redman, J.W., Butler, J.M., Boyer, D.A. (2002) Polymerse chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the “suitability for purpose” of four filter papers as archival media. Anal. Chem. 74: 1863-1869.

 

Vallone, P.M., Devaney, J.M., Marino, M.A., Butler, J.M. (2002) A strategy for examining complex mixtures of deoxyoligonucleotides using IP-RP HPLC, MALDI-TOF MS, and informatics. Anal. Biochem. 304: 257-265.

 

Butler, J.M., Schoske, R., Vallone, P.M., Kline, M.C., Redd, A.J., Hammer, M.F. (2002) A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers. Forensic Sci. Int. 129: 10-24.

 

Butler, J.M., David, V.A., O’Brien, S.J., Menotti-Raymond, M. (2002) The MeowPlex: a new DNA test using tetranucleotide STR markers for the domestic cat. Profiles in DNA, Promega Corporation, Volume 5, No. 2, pp. 7–10. http://www.promega.com/profiles/502/ProfilesInDNA_502_07.pdf

 

Redd, A.J., Agellon, A.B., Kearney, V.A., Karafet, T., de Knijff, P., Park, H., Butler, J.M., Hammer, M.F. (2002) Forensic value of fourteen novel STRs on the human Y chromosome. Forensic Sci. Int. 130: 97-111.

2001

Butler, J.M. (2001) Forensic DNA Typing: Biology and Technology behind STR Markers, Academic Press, London.

 

Ruitberg, C.M., Reeder, D.J., Butler, J.M. (2001) STRBase: a short tandem repeat DNA database for the human identity testing community. Nucleic Acids Res. 29: 320-322.

 

McCord, B.R. and Butler, J.M. (2001) The application of capillary electrophoresis to the analysis of PCR products used in DNA typing. Methods in Molecular Biology: Clinical and Forensic Applications of Capillary Electrophoresis (Petersen, J. and Mohammad, A.A., eds.), Humana Press: Totowa, New Jersey, pp. 261-284.

 

Butler, J.M. and Reeder, D.J. (2001) Detection of DNA polymorphisms using PCR-RFLP and capillary electrophoresis.  Methods in Molecular Biology: Capillary Electrophoresis of Nucleic Acids (Mitchelson, K.R. and Cheng, J., eds.), Humana Press: Totowa, New Jersey, Vol. 2, pp. 49-56.

 

Butler, J.M., Devaney, J.M., Marino, M.A., Vallone, P.M. (2001) Quality control of PCR primers used in multiplex STR amplifications. Forensic Sci. Int., 119: 87-96.

 

Butler, J.M., C.M. Ruitberg, Vallone, P.M. (2001) Capillary electrophoresis as a tool for optimization of multiplex PCR reactions, Fresenius J. Anal. Chem. 369: 200-205.

 

Devaney, J.M. Pettit, E.L., Kaler, S.G., Vallone, P.M., Butler, J.M., Marino, M.A. (2001) Genotyping of two mutations in the HFE gene using single-base extension and high-performance liquid chromatography. Anal. Chem. 73: 620-624.

 

Butler, J.M. and Becker, C.H. (2001) Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry.  Final Report for NIJ Grant 97-LB-VX-0003, Office of Justice Programs, National Institute of Justice (75 published pages); http://www.ojp.usdoj.gov/nij/pubs-sum/188292.htm

 

Schoske, R., Butler, J.M., Vallone, P.M., Kline, M.C., Prinz, M., Redd, A.J., Hammer, M.F. (2001) Development of Y STR megaplex assays. Proceedings of the Twelve International Symposium on Human Identification 2001, Promega Corporation. http://www.promega.com/geneticidproc/ussymp12proc/contents/butler.PDF

 

Duewer DL, Kline MC, Redman JW, Newall PJ, Reeder DJ. (2001) NIST Mixed Stain Studies #1 and #2: interlaboratory comparison of DNA quantification practice and short tandem repeat multiplex performance with multiple-source samples.  J. Forensic Sci. 46: 1199-1210

2000

Butler, J.M. (2000) MALDI-TOF mass spectrometry. Progress in Forensic Genetics 8 (Sensabaugh, G.F., Lincoln, P.J., and Olaisen, B., eds.), Elsevier Science: Amsterdam, The Netherlands, pp. 15-18.

 

Duewer DL, Richie KL, Reeder DJ. (2000) RFLP band size standards: NIST Standard Reference Material® 2390.  J. Forensic Sci. 45: 1093-1105.

 

Duewer DL, Gary KT, Reeder DJ. (2000) RFLP band size standards: Cell line K562 values from 1991 – 1997 proficiency studies.  J. Forensic Sci. 45: 1106-1118.

1999

Schwarz, F.P., Robinson, S., Butler, J.M. (1999) Thermodynamic comparison of PNA/DNA and DNA/DNA hybridization reactions at ambient temperature. Nucleic Acids Res. 27: 4792-4800.

 

Butler, J.M. and Reeder, D.J. (1999) Capillary electrophoretic separation of DNA fragments. Nucleic Acid Electrophoresis (Tietz, D., ed.), Springer-Verlag: Berlin, Germany, Chapter 7, pp. 170-192.

 

Li, J., Butler, J.M., Tan, Y., Lin, H., Royer, S., Ohler, L., Shaler, T.A., Hunter, J.M., Pollart, D.J., Monforte, J.A., Becker, C.H. (1999) Single nucleotide polymorphism determination using primer extension and time-of-flight mass spectrometry. Electrophoresis 20:1258-1265.

1998

Butler, J.M., Li, J., Shaler, T.A., Monforte, J.A., Becker, C.H. (1998) Reliable genotyping of short tandem repeat loci without an allelic ladder using time-of-flight mass spectrometry. Int. J. Legal Med. 112: 45-49.

Presentations (click on .pdf link to download a handout of the presentation)

[2005] [2004] [2003] [2002] [2001] [2000]

NOTE: some information may have been removed prior to publication

2005

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), January 18, 2005, “NIST Research Update” [.pdf]

John Butler talk at Myriad Genetics (Salt Lake City, UT), February 3, 2005, "Efforts towards Standardization of Validation and Other NIST Research Projects"

John Butler seminar for BYU Department of Chemistry and Biochemistry (Provo, UT), February 4, 2005, "Forensic DNA Typing: Projects, Perspectives, and Potential"

Mike Coble talk at 57^th Annual Meeting of the American Academy of Forensic Sciences (New Orleans, LA), February 25, 2005, "Genotyping of Nuclear Loci from Telogenic Hair Shafts using mini-STRs" [.pdf]

Peter Vallone talk at 57^th Annual Meeting of the American Academy of Forensic Sciences (New Orleans, LA), February 26, 2005, "Evaluation of SNPs as Tools in Human Identity Testing" [.pdf]

Margaret Kline talk at 57^th Annual Meeting of the American Academy of Forensic Sciences (New Orleans, LA), February 25, 2005, "Some Interesting Point Mutations and Deletions Found Through STR Allele Sequencing" [.pdf]

John Butler talk at 229th American Chemical Society National Meeting special session on Finding Criminals with Forensic Chemistry (San Diego, CA), March 14, 2005, "Advances in the Biochemistry of DNA Fingerprinting Analysis" [.pdf]

Margaret Kline talk at Mini-Symposium: Dry-State Storage of DNA (Frederick, MD), March 29, 2005, "Recovery of DNA from Aged Bloodstains" [.pdf]

Mike Coble talk at EDNAP (European DNA Profiling Group) meeting (Glasgow, Scotland), April 4, 2005, "NIST Research Update" [.pdf]

Margaret Kline talk at Present and Future Technological Advances in Human Identification meeting (Charlottesville, VA), April 5, 2005, "NIST Research Update" [.pdf]

Mike Coble talk at ENFSI (European Network of Forensic Science Institutes) meeting (Glasgow, Scotland), April 6, 2005, "NIST Research Update" [.pdf]

Mike Coble talk for Strathclyde Police DNA Laboratory (Glasgow, Scotland), April 6, 2005, "NIST Research Update" [.pdf]

John Butler talk for CCQM (Consultative Committee for Amount of Substance) Bioanalysis Working Group (Sèvres, France), April 12, 2005, "DNA Profiling and Quantitation of Human DNA" [.pdf]

David Duewer talk for CCQM (Consultative Committee for Amount of Substance) Workshop on "New Challenges for the Development of Primary or Higher Order Measurement Methods and Procedures for Physiologically-Significant Molecules" (Sèvres, France), April 13, 2005, "Human Genomic Quantitation Based on Chromosome Counting"

John Butler talk for Human Identification E-Symposium (internet broadcast), April 14, 2005, "A Primer on DNA Profiling Using STR Markers" [.pdf] (see http://www.humid.e-symposium.com/speaker-program.php)

Mike Coble talk at NIH Mitochondrial Molecular Biology and Pathology Workshop (Bethesda, MD), April 29, 2005, "Forensic Application of Sequence Variation in the Human mtDNA Genome" [.pdf]

John Butler, Mike Coble, Peter Vallone, and Margaret Kline talk to the FBI Laboratory (Quantico, VA), May 19, 2005, "NIST Projects in Human Identity Testing" [.pdf]

Margaret Kline talk at Bode East Coast Advanced DNA Technology Workshop (Duck Key, FL), May 24, 2005, "Finding Point Mutations, Deletions and New Alleles Through STR Allele Sequencing" [.pdf]

John Butler talk at Canadian Forensic DNA Technology Workshop (Toronto, Ontario), June 8, 2005, "State of the Y Chromosome: New Advances and State of the Science for Y Chromosome DNA Testing" [.pdf]

John Butler talk at Canadian Forensic DNA Technology Workshop (Toronto, Ontario), June 8, 2005, "Capillary Electrophoresis Instrumentation and STR Kits" [.pdf]

John Butler and Bruce McCord workshop at University of Albany DNA Academy (Albany, NY), June 13-14, 2005 [STR Analysis] [Intro to CE and ABI 310] [Validation and Interlaboratory Studies] [Real-time qPCR and miniSTRs] [Statistics and Higher Throughput Approaches for STR Typing] [Y-Chromosome Analysis] [Troubleshooting CE Systems]

David Duewer and John Butler demonstration and posters at NIJ DNA Grantees meeting (Washington, D.C.), June 28, 2005, "Multiplex_QA: An Exploratory Quality Assessment Tool for STR Multiplex Assays" [.pdf] and "New Tools to Aid Work with STR Profile Mixtures: mixSTR and Virtual MixtureMaker" [.pdf]

John Butler talk at NIJ DNA Grantees meeting (Washington, D.C.), June 29, 2005, "NIST On-Going Projects to Aid the Human Identity Testing Community" [.pdf]

Margaret Kline talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), June 30, 2005, "NIST Research Update" [.pdf]

John Butler presentation to Applied Biosystems (Foster City, CA), July 19, 2005, "NIST On-Going Projects to Aid the Human Identity Testing Community" [.pdf]

Peter Vallone talk at Association of Forensic DNA Analysts and Administrators summer meeting (Austin, TX), August 4, 2005, "NIST Projects in Human Identity Testing" [.pdf]

2004

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), January 8, 2004, “NIST Research Update” [.pdf]

John Butler talk at NIST as part of Sigma Xi lecture series (Gaithersburg, MD), January 13, 2004, "Technology Developments in Forensic DNA Typing" [.pdf]

John Butler talk at American Academy of Forensic Sciences workshop on Forensic Human Mitochondrial DNA Analysis (Dallas, TX), February 16, 2004, "SNPs and Strips: Approaches to Rapid Screening of mtDNA Types" [.pdf]

John Butler talk at American Academy of Forensic Sciences workshop on Y-STR Analysis on Forensic Casework (Dallas, TX), February 17, 2004, "Going Beyond the U.S. Haplotype: A Look at Additional Y-STR and Y-SNP Loci in U.S. Populations" [.pdf]

Mike Coble talk at 56^th Annual Meeting of the American Academy of Forensic Sciences (Dallas, TX), February 20, 2004, "Development of New miniSTR Loci for Improved Analysis of Degraded DNA Samples" [.pdf]

John Butler, Peter Vallone, Margaret Kline, and Mike Coble presentations at National Institute of Justice DNA Program Review (Washington, DC), March 25-26, 2004 and April 1-2, 2004, "Forensic DNA Typing Projects Conducted by NIST Human Identity Testing Project Team in FY2004"

John Butler keynote talk at MEMS Alliance Symposium (Laurel, MD), March 29, 2004, “Technology Developments in Forensic DNA Typing”

Margaret Kline talk at Seventh Annual STR Megaplex Advanced Research and Training Workshop (Virginia Beach, VA), March 29, 2004, "Capillary Electrophoresis 101: the ABI 3100" [.pdf]

Margaret Kline talk at Seventh Annual STR Megaplex Advanced Research and Training Workshop (Virginia Beach, VA), March 30, 2004, "Alternatives to Quantification: Real-Time PCR" [.pdf]

John Butler talk at EDNAP (European DNA Profiling Group) meeting (Nicosia, Cyprus), April 15, 2004, "Forensic DNA Projects at NIST" [.pdf]

John Butler talk at ENFSI (European Network of Forensic Science Institutes) DNA Working Group meeting (Nicosia, Cyprus), April 16, 2004, "Forensic DNA Projects at NIST" [.pdf]

John Butler talk at Mitretek Systems' Biometrics Identification Seminar (Falls Church, VA), May 12, 2004, "Forensic DNA Typing and Prospects for Biometrics" [.pdf] [PowerPoint]

John Butler talk at CODIS State Administrators meeting (Quantico, VA), May 25, 2004, "The Future of Forensic DNA Typing: Y-STRs, SNPs, and Real-time PCR" [.pdf]

Peter Vallone poster at CHI Nucleic Acids Based Technologies meeting (McLean, VA), June 21-23, 2004, "AutoDimer: a rapid screening tool for primer dimer and hairpin structures"

Peter Vallone and Margaret Kline talk at NIJ DNA Grantees meeting (Washington, DC), June 30, 2004, "New Autosomal Forensic Markers and a Review of the NIST Interlaboratory Quantitation Study" [.pdf]

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (Wingate Inn, Stafford, VA), July 12, 2004 "NIST Research Update" [.pdf]

John Butler, Peter Vallone, and Michael Coble talk at Armed Forces DNA Identification Laboratory (Rockville, MD), August 3, 2004 "NIST Research Summary for AFDIL" [.pdf]

Margaret Kline talk at the Community and Technical College of Shepherd (Martinsburg, WV), September 28, 2004 "Forensic DNA Identification and Standards Development" [.pdf]

John Butler and Bruce McCord workshop at Northeastern Association of Forensic Scientists (Mystic, CT), September 29-30, 2004 "Capillary Electrophoresis in DNA Analysis" [STR Analysis][Intro to CE][Data Interpretation][RT-PCR and miniSTRs][Higher Throughput Approaches][Troubleshooting the ABI 310][Additional Topics]

John Butler talk at 15th International Symposium on Human Identification (Phoenix, AZ), October 6, 2004 "Can the Validation Process in Forensic DNA Typing Be Standardized?" [.pdf]

John Butler talk at 10th CODIS Conference (Crystal City, VA), November 15, 2004 "NIST Update" [.pdf]

Mike Coble talk at The International Symposium on the Application of DNA Technologies in Analytical Sciences held at Fooyin University (Kaohsiung, Taiwan), November 16, 2004 "Development of new miniSTR loci for improved analysis of degraded DNA samples" [.pdf]

Mike Coble talk at the Taipei Symposium on the Application of mtDNA Technology in Forensic Science held at the Central Police University (Taipei, Taiwan), November 18, 2004 [.pdf]

John Butler talk at IV. International Forensic Y-User Workshop-Haploid DNA markers in forensic genetics (Berlin, Germany), November 20, 2004 "Y-chromosome and mitochondrial DNA work at the U.S. National Institute of Standards and Technology" [.pdf]

2003

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), January 14, 2003, “Y Chromosome Work at NIST”[.pdf]

Margaret Kline talk at 55^th Annual Meeting of the American Academy of Forensic Sciences (Chicago, IL), February 20, 2003, "NIST Mixed Stain Study #3: Does DNA Concentration Measurement Accuracy Matter?" [.pdf]

Peter Vallone talk at George Washington University Department of Biological Sciences (Washington, DC), February 28, 2003, “Development of Multiplexed Assays for Evaluating SNP and STR Forensic Markers “ [.pdf]

John Butler talk at Rockville Police Academy (Rockville), March 13, 2003, “Introduction to Forensic DNA Typing" [.pdf]

Margaret Kline talk at Sixth Annual Fluorescent STR MegaPlex Technology Workshop (Virginia Beach, VA), March 24, 2003, "Polyacrylamide Gel Electrophoresis 101" [.pdf]

Margaret Kline talk at Sixth Annual Fluorescent STR MegaPlex Technology Workshop (Virginia Beach, VA), March 24, 2003, "Hitachi FMBIO IIe and FMBIO III Comparisons Issues" [.pdf]

John Butler talk at Forensic Applications of Y-Chromosome DNA Typing Symposium (Nashville, TN), May 12, 2003, “NIST Y Chromosome Standards and Multiplex Assays” [.pdf]

Peter Vallone talk at NIST 4^th Annual Biotechnology Division Retreat (Shepherdstown, WV), May 15, 2003, “An Allele-Specific Primer Extension Assay for the Detection of 11 SNPs Located in the Mitochondrial Genome” [.pdf]

John Butler talk at BIO2003 meeting (Washington DC), June 23, 2003, “When a Life is in the Balance… Your Standards and Technology Better be Good: Human Identity Testing at NIST” [.pdf]

John Butler talk at NIJ DNA Grantees meeting (Washington DC), June 23, 2003, “NIST Y Chromosome Work and Standard Reference Material SRM 2395” [.pdf]

Margaret Kline talk at NIJ DNA Grantees meeting (Washington DC), June 23, 2003, “How to Make NIST Traceable Materials" [.pdf]

Peter Vallone talk at NIJ DNA Grantees meeting (Washington DC), June 23, 2003, “Development of Multiplexed SNP Assays from Mitochondrial and Y Chromosome DNA for Human Identity Testing" [.pdf]

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), July 17, 2003, “NIST Research Update” [.pdf]

Peter Vallone talk to Summer Undergraduate Research Fellowship (SURF) students at NIST (Gaithersburg, MD), July 25, 2003, “Forensic DNA Typing:  Application to Mass Disaster Investigations, Paternity Testing and Human Identification” [.pdf]

Margaret Kline talk at 2003 NCSL International Workshop and Symposium (Tampa, FL), August 18, 2003, "Biotech SRMs Designed for the Rapidly Evolving Forensic DNA and Human Identity Testing Communities" [.pdf]  

John Butler talk at Relative Genetics (Salt Lake City, UT), August 29, 2003, "NIST Developments in Y-Chromosome Analysis" [.pdf]

John Butler talk at Myriad Genetics (Salt Lake City, UT), August 29, 2003, "NIST Developments in Y-Chromosome Analysis" [.pdf]

John Butler poster at International Society of Forensic Genetics - 20th International Congress ISFG (Arcachon, France), September 9-13, 2003, "Forensic Value of the Multi-Copy Y-STR Marker DYS464" [.pdf]

Peter Vallone talk at International Society of Forensic Genetics - 20th International Congress ISFG (Arcachon, France), September 12, 2003, "Multiplexed Assays for Evaluation of Y SNP Markers in U.S. Populations" [.pdf]

Margaret Kline talk at Public SWGDAM meeting held in conjunction with 14th International Symposium on Human Identification (Phoenix, AZ), September 28, 2003, "NIST Research Update" [.pdf]

Margaret Kline poster at 14th International Symposium on Human Identification (Phoenix, AZ), September 30-October 2, 2003, "Semi-Automation of mtDNA Arrays: Results from 666 Population Samples and Comparisons" [.pdf]

Margaret Kline poster at 14th International Symposium on Human Identification (Phoenix, AZ), September 30-October 2, 2003, "NIST SRM 2395 and Other Y Chromosome Work" [.pdf]

Peter Vallone talk for NIST Biotechnology Division Seminar (Gaithersburg, MD), October 16, 2003, "Typing Single Nucleotide Polymorphisms (SNPs) Located on the Y Chromosome and in the Mitochondrial Genome" [.pdf]

Peter Vallone talk at the Royal Institute of Technology, Department of Biotechnology (Stockholm, Sweden), November 18, 2003, "Multiplex SNP Assays for the Evaluation of Forensic Markers" [.pdf]

Peter Vallone talk at Uppsala University, Department of Genetics and Pathology (Uppsala, Sweden), November 19, 2003, "A Multiplex Primer Extension Assay for Probing 11 SNPs Located in the Mitochondrial Genome" [.pdf]

 

2002

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), January 16, 2002, "MiniSTR Development to Aid Testing of Degraded Samples" [.pdf] 

John Butler talk at Scientific Working Group on DNA Analysis Methods SWGDAM (FBI Academy, Quantico, VA), January 16, 2002, "NIST Research Update" [.pdf]

John Butler talk at NCI-Frederick to Cat DNA Advisory Committee (Frederick, MD), January 28, 2002, "Cat STR Multiplex Development" [.pdf] 

Peter Vallone talk at Forensic Mitochondrial DNA Analysis: A Community Forum Workshop for American Academy of Forensic Sciences (Atlanta, GA), February 11, 2002, "Analyzing Single Nucleotide Polymorphisms" [.pdf]

John Butler talk at Y Chromosome Workshop for American Academy of Forensic Sciences (Atlanta, GA), February 12, 2002, "Recent Developments in Y-Chromosome Analysis of Y-STRs and Y-SNPs" [.pdf]

Margaret Kline talk at American Academy of Forensic Sciences (Atlanta, GA), February 14, 2002 , "Effects of Storage Temperature and Humidity Control on the Recovery of DNA from Aged-Bloodstains" [.pdf]

John Butler talk at 3rd World Trade Center Kinship and Data Analysis Panel-WTC KADAP (Albany, NY), February 22, 2002, "MiniSTR Development to Aid Testing of Degraded DNA Samples" [.pdf]

Margaret Kline talk at 5th Annual STR MegaPlex and Research Technology Workshop (Hilton Head, SC), March 10-15, 2002, "NIST Mixed Stain Study #3 The 2001 Interlaboratory Challenge Exercise" [.pdf]

Margaret Kline talk at 5th Annual STR MegaPlex and Research Technology Workshop (Hilton Head, SC), March 10-15, 2002, "Capillary Electrophoresis 101, the ABI 310/3100" [.pdf] 

Margaret Kline talk at 5th Annual STR MegaPlex and Research Technology Workshop (Hilton Head, SC), March 10-15, 2002, "Recovery of DNA from Aged Bloodstains on Untreated Paper" [.pdf] 

Margaret Kline talk at 5th Annual STR MegaPlex and Research Technology Workshop (Hilton Head, SC), March 10-15, 2002, "Y-STRs and the International Scene" [.pdf]

John Butler talk at Mid-Atlantic Association of Forensic Scientists annual meeting (Frederick, MD), April 25, 2002, "New DNA tests for improving analysis of degraded DNA and male-female mixtures" [.pdf]

John Butler talk (Keynote Address for Biological Criminalistics Section) at 16th International Symposium on the Forensic Sciences (Canberra, Australia), May 13, 2002, "Developing New Technologies in Forensic DNA Typing" [.pdf]

John Butler talk at SNP Workshop for 16^th International Symposium on the Forensic Sciences (Canberra, Australia), May 15, 2002, "Technologies for SNP Typing with Applications in Mitochondrial and Y Chromosome Markers" [.pdf]

John Butler talk at American Chemical Society Mid-Atlantic Regional Meeting (Fairfax, VA), May 28, 2002, "Overview, history, and issues with forensic DNA testing" [.pdf]

Richard Schoske talk at American Chemical Society Mid-Atlantic Regional Meeting (Fairfax, VA), May 28, 2002, "Y chromosome STR markers and the development of Y STR multiplex PCR assays" [.pdf]

Peter Vallone poster at 50^th Annual Meeting of the American Society of Mass Spectrometry (Orlando, FL), June 2-6, 2002, "Rapid genotyping of Y chromosome SNPs using a UV photocleavable oligonucleotide in MALDI-TOF MS" [.pdf]

John Butler and Margaret Kline talk at National Institute of Justice DNA Grantees Meeting (Washington, DC), June 25, 2002, "Multiplex assay development for mitochondrial and Y chromosome DNA testing" [.pdf] 

Gordon Spangler talk at National Institute of Justice DNA Grantees Meeting (Washington, DC), June 25, 2002, "Development of Y SNP Assays for a Multi-Color Fluorescence Detection Platform" [.pdf]  

Peter Vallone talk at Cambridge Healthtech Institute's 5th Annual DNA Forensics Meeting (Washington, DC), June 28, 2002, "Multiplexed Assays for Probing Y Chromosome and Mitochondrial Markers" [.pdf]

Peter Vallone talk at NCI-Advanced Technology Center (Gaithersburg, MD), July 10, 2002, "Development of Multiplexed Assays for Typing SNP and STR Forensic Markers" [.pdf]

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), July 2002, "NIST Research Update" [.pdf]

John Butler talk at 6th WTC Kinship and Data Analysis Panel (New York City, NY), September 9, 2002, "NIST Concordance Study of Orchid SNP Markers" [.pdf]

John Butler talk at Workshop on the Y Chromosome for 13^th International Symposium on Human Identification (Phoenix, AZ), October 9, 2002; “The Human Y Chromosome: A Final Frontier in Human Identity Testing” [.pdf]

Peter Vallone poster at 52^nd Annual American Society of Human Genetics meeting (Baltimore, MD), October 2002, "Multiplex Detection of 10 SNPs Located in the Coding Region of the Mitochondrial Genome” [.pdf]

John Butler talk at the 3^rd International Forensic Y-User Workshop, Porto, Portugal, November 7, 2002, “NIST Y Chromosome Standards and Multiplex Assays” [.pdf]

2001

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), January 23, 2001, "Update on NIST Activities Regarding Forensic DNA Typing" [.pdf]

John Butler talk at American Type Culture Collection-ATCC (Manassas, VA), February 1, 2001, "Genotyping DNA Samples for Human Identity Testing Purposes" [.pdf]

John Butler talk at 53^rd Annual Meeting of the American Academy of Forensic Sciences (Seattle, WA), February 22, 2001, "Comparison of Primer Sequences Used in Commercial STR Kits" [.pdf]

John Butler talk at Florida International University Department of Chemistry (Miami, FL), March 9, 2001, "Developing New Technology for Forensic DNA Typing" [.pdf] 

Margaret Kline talk at Marine Genomics Consortium, NIST Charleston Laboratory (Charleston, SC), March 9, 2001, "Overview of the NIST CSTL Biotechnology Division" [.pdf]

Margaret Kline talk at STR Megaplex Advanced Research and Training Workshop (Hilton Head, SC), March 13, 2001, "NIST Update on SRM Availability and New Projects" [.pdf]

Margaret Kline talk at STR Megaplex Advanced Research and Training Workshop (Hilton Head, SC), March 14, 2001, "NIST Update: What Have We Learned about DNA Stability and Quantitiation?" [.pdf]

John Butler talk at Marshall University (Huntington, WV), April 2, 2001, "DNA Typing by Mass Spectrometry" [.pdf]

John Butler and Peter Vallone talk at NIST Centennial Open House (Gaithersburg, MD), May 11, 2001, "Identifying Criminals with DNA" [.pdf]  

Peter Vallone talk at Bruker Daltonics Annual User Meeting held as part of the American Society of Mass Spectrometry Meeting (Chicago, IL), May 27, 2001, "DNA Mass Spectrometry at NIST" [.pdf]

Peter Vallone poster at American Society of Mass Spectrometry (Chicago, IL), May 27, 2001, "DNA Mass Spectrometry at NIST" [.pdf]

John Butler talk at NIJ DNA Grantees Meeting (Washington, DC), June 7, 2001, "Multiplex SNP Assays for mtDNA and Y Chromosome Markers Using Time-of-Flight Mass Spectrometry" [.pdf]

John Butler and Peter Vallone talk at FBI Headquarters to DNA Units (Washington, DC), June 12, 2001, "Developing New DNA Tests Using Y Chromosome Markers" [.pdf]

John Butler talk at LGC (Teddington, England), June 19, 2001, "High-throughput genotyping and work with human identity testing at NIST" [.pdf]

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), July 12, 2001, "Update on NIST activities with forensic DNA typing" [.pdf]

John Butler talk at 19^th Congress of the International Society of Forensic Genetics (Munster, Germany), August 30, 2001, "Highly multiplexed assays for measuring polymorphisms on the Y-chromosome" [.pdf]

John Butler talk at ABI 310 Workshop for the Midwestern Association of Forensic Sciences (Minneapolis, MN), September 24, 2001, "Short tandem repeat DNA typing systems" [.pdf]

John Butler talk at ABI 310 Workshop for the Midwestern Association of Forensic Sciences (Minneapolis, MN), September 24, 2001, "ABI Prism 310 Genetic Analyzer: Chemistry, Hardware, and Software" [.pdf]

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM Public Meeting (Biloxi, MS), October 8, 2001, "Preliminary Results from the 2001 NIST Mixed-Stain Interlaboratory Study #3: DNA Quantitation and Identification of Minor Contributor(s)" [.pdf]

Margaret Kline poster at Twelve International Symposium on Human Identification (Biloxi, MS), October 10-11, 2001, "Preliminary Results from the 2001 NIST Mixed-Stain Interlaboratory Study #3: DNA Quantitation and Identification of Minor Contributor(s)" [.pdf]

Peter Vallone poster at Twelve International Symposium on Human Identification (Biloxi, MS), October 10-11, 2001, "Genotyping single nucleotide polymorphisms in the Y chromosome and the mitochondrial genome" [.pdf]

Jan Redman poster at Twelve International Symposium on Human Identification (Biloxi, MS), October 10-11, 2001, Recovery of DNA from aged-bloodstains on un-treated paper" [.pdf]

Margaret Kline poster at Twelve International Symposium on Human Identification (Biloxi, MS), October 10-11, 2001, "How to make NIST traceable materials" [.pdf]

John Butler poster at Twelve International Symposium on Human Identification (Biloxi, MS), October 10-11, 2001, "STR Typing with the 16-capillary ABI 3100 Genetic Analyzer" [.pdf] 

John Butler poster at Twelve International Symposium on Human Identification (Biloxi, MS), October 10-11, 2001, "Prototype Y Chromosome Standard (NIST SRM 2395)" [.pdf]

Richard Schoske talk at Twelve International Symposium on Human Identification (Biloxi, MS), October 12, 2001, "Developing Y STR megaplexes" [.pdf]

Peter Vallone poster at American Society of Human Genetics Meeting (San Diego, CA), October 2001, "SNP Analysis" [.pdf]

John Butler lecture for George Mason University STR Course (Manassas, VA), October 25, 2001, "Technology Developments for DNA Typing" [.pdf]

 

2000

Margaret Kline talk at Scientific Working Group on DNA Analysis Methods-SWGDAM (FBI Academy, Quantico, VA), January 10, 2000, "Update on NIST Activities in DNA Forensics" [.pdf]    

John Butler at NIJ Grantees Meeting (Reno, NV), February 21, 2000, "NIJ-Funded Research Effort at NIST and Wrap-up on GeneTrace Grant" [.pdf]

Margaret Kline talk at American Academy of Forensic Sciences meeting (Reno, NV), February 26, 2000, "Documenting Real-World Pipette Performance" [.pdf]

John Butler talk at NIJ First Annual DNA Grantees Meeting (Washington, DC), March 7, 2000, "Technology Developments for DNA Typing" [.pdf]

Margaret Kline talk at Third Annual Fluorescent STR MegaPlex Technology Workshop (Hilton Head, SC), March 12-18, 2000, "Documenting Real-World Pipette Performance" [.pdf]

Margaret Kline talk at Third Annual Fluorescent STR MegaPlex Technology Workshop (Hilton Head, SC), March 12-18, 2000, "Quantification of DNA Issues and Recommendations, Observations from the 1999 NIST Mixed-Stain Study #2" [.pdf] 

John Butler talk at University of Virginia Department of Chemistry (Charlottesville, VA), March 24, 2000, "Short Tandem Repeat DNA Markers and Their Use in DNA Typing" [.pdf]

John Butler talk at MWG Biotech SNP Seminar (Bethesda, MD), March 31, 2000, "Development of Multiplex SNP Assays Involving Primer Extension and Time-of-Flight Mass Spectrometry" [.pdf]

John Butler talk at Cambridge Healthtech Institute's Fourth Annual DNA Forensics Conference (Springfield, VA), June 1, 2000, "Y Chromosome DNA Variation Monitored by SNP and STR Analysis" [.pdf]

John Butler talk at NIJ's Crime Laboratory Improvement Program Summit (Washington, DC), July 14, 2000, "Technology Transfer Issues" [.pdf]

Peter Vallone and John Butler talk at FBI Laboratory's Forensic Science Research Unit (FBI Academy, Quantico, VA), September 8, 2000, "NIST Research Projects Related to Forensic DNA Typing" [.pdf]

John Butler talk at Scientific Working Group on DNA Analysis Methods-SWGDAM Public Meeting (Biloxi, MS), October 8, 2000, "Update on NIST activities regarding forensic DNA typing" [.pdf]

John Butler poster at Eleventh International Symposium on Human Identification (Biloxi, MS), October 11-12, 2000, "STRBase: update on the NIST forensic STR database" [.pdf] 

John Butler poster at Eleventh International Symposium on Human Identification (Biloxi, MS), October 11-12, 2000, "New Primer Sets for Y Chromosome and CODIS STR Loci" [.pdf]

Peter Vallone poster at Eleventh International Symposium on Human Identification (Biloxi, MS), October 11-12, 2000, "DNA Mass Spectrometry at NIST" [.pdf]

John Butler talk at Eleventh International Symposium on Human Identification (Biloxi, MS), October 13, 2000, "Multiplex PCR and Determination of Primer Sequences Used in STR Kits" [.pdf]

John Butler talk at NIST Biotechnology Division Seminar (Gaithersburg, MD), December 13, 2000, "High-Throughput Genotyping and Applications in Human Identity Testing" [.pdf] 

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